Table 1.

Formation time, morphology, and intensity of callus from Justicia gendarussa Burm. f., with variation in sucrose concentration for 6 weeks in dark and light conditions.

Week	Sucrose concetration (%)	Dark					Light
		Eficiency of callus initiation (%)	Callus formation time (week)	Callus morphology		Callus intensity	Eficiency of callus initiation (%)	Callus formation time (week)	Callus morphology		Callus intensity
				Colour	Texture				Colour	Texture
1	0	–	–	–	–	–	–	–	–	–	–
	10	–	–	–	–	–	–	–	–	–	–
	20	–	–	–	–	–	–	–	–	–	–
	30	–	–	–	–	–	–	–	–	–	–
	40	–	–	–	–	–	–	–	–	–	–
	50	–	–	–	–	–	–	–	–	–	–
2	0	–	–	–	–	–	–	–	–	–	–
	10	100	2	White	Friable	+	100	2	White	Friable	+
	20	100	2	White	Friable	+	100	2	White	Friable	+
	30	100	2	White	Friable	+	100	2	White	Friable	+
	40	100	2	White	Friable	+	100	2	White	Friable	+
	50	100	2	White	Friable	+	100	2	White	Friable	+
3	0	–	–	–	–	–	100	3	White	Friable	+
	10	100	2	White	Friable	+	100	2	White	Friable	+
	20	100	2	Yellowish	Compact	++	100	2	Yellowish	Compact	++
	30	100	2	Yellowish	Compact	++	100	2	Yellowish	Compact	++
	40	100	2	Yellowish	Compact	++	100	2	Yellowish	Compact	++
	50	100	2	Brown	Compact	++	100	2	Brownish	Compact	++
4	0	–	–	–	–	–	100	3	White	Friable	+
	10	100	2	White	Friable	+	100	2	White	Friable	+
	20	100	2	Yellowish	Compact	+++	100	2	Yellowish	Compact	+++
	30	100	2	Yellowish	Compact	++++	100	2	Yellowish	Compact	++++
	40	100	2	Muddy white	Compact	++++	100	2	Muddy white	Compact	++++
	50	100	2	Brown	Compact	++++	100	2	Brownish	Compact	++++
5	0	–	–	–	–	–	100	3	Yellowish	Compact	++
	10	100	2	Yellowish	Compact	++	100	2	Yellowish	Compact	++
	20	100	2	Brownish	Compact	+++	100	2	Muddy white	Compact	++++
	30	100	2	Brownish	Compact	++++	100	2	Brownish	Compact	++++
	40	100	2	Brownish	Compact	++++	100	2	Brown	Compact	++++
	50	100	2	Dark brown	Compact	++++	100	2	Brown	Compact	++++
6	0	–	–	–	–	–	100	2	Brown	Compact	++
	10	100	2	Dark brown	Compact	+++	100	2	Dark brown	Compact	++++
	20	100	2	Dark brown	Compact	++++	100	2	Dark brown	Compact	++++
	30	100	2	Dark brown	Compact	++++	100	2	Dark brown	Compact	++++
	40	100	2	Dark brown	Compact	++++	100	2	Dark brown	Compact	++++
	50	100	2	Dark brown	Compact	++++	100	2	Dark brown	Compact	++++

Note: -, no callus is formed; +, 25 % callus of explants; ++, 50 % callus of explants; +++, 75 % callus of explants; ++++, 100 % callus of explant.