CELL BIOLOGY Correction for “Statin-induced GGPP depletion blocks macropinocytosis and starves cells with oncogenic defects,” by Zhihua Jiao, Huaqing Cai, Yu Long, Orit Katarina Sirka, Veena Padmanaban, Andrew J. Ewald, and Peter N. Devreotes, which was first published February 12, 2020; 10.1073/pnas.1917938117 (Proc. Natl. Acad. Sci. U.S.A. 117, 4158–4168).
The authors note that, due to a duplication error, the image of PTEN−/− cells treated with 3µM pitavastatin without the addition of BSA in Fig. 6B is the same as the image in Fig. 6A, when PTEN−/− cells are treated with 4µM pitavastatin. The corrected figure and its legend appear below.
Fig. 6.
Defective macropinocytosis after pitavastatin induces protein and amino acid starvation in PTEN−/− cells. (A) The sensitivity of pitavastatin increases in PTEN−/− cells under serum-free conditions. (Scale bar, 30 μm.) (B) BSA rescues the cytotoxic effects of pitavastatin in mammalian PTEN−/− cells under serum-free conditions. (Scale bar, 30 μm.) (C) Total intracellular BSA in MCF10A and PTEN−/− cells increases with increasing concentrations of BSA in the medium (mean ± SD, n = 3). (D) Leucine (Leu) rescues the cytotoxic effects of pitavastatin on mammalian PTEN−/− cells under serum-free conditions. (Scale bar, 30 μm.) (E) Fluid-phase uptake in Dictyostelium pten− cells in HL5 medium and FM medium. pten− cells in HL5 medium were added with 1 μM pitavastatin; pten− cells in FM medium were added with 4 μM pitavastatin. (Scale bar, 20 μm.) (F) Measurement of fluid-phase uptake in Dictyostelium pten− cells in HL5 medium and FM medium (n = 3 experiments, nonparametric Mann–Whitney–Wilcoxon test, ****P < 0.0001). (G) Dictyostelium pten− cells show resistance to pitavastatin in FM medium containing abundant amino acids. (Scale bar, 20 μm.) (H) Proposed molecular architecture of the mevalonate pathway and macropinocytosis.