Fig. 1.

Three proteins, McpA, PopZ, and SpmX, in C. crescentus imaged with two different imaging modalities. (A, Top) Tomographic slices of CET data showing the two different cell types, motile swarmer and sessile stalked, of C. crescentus. (A, Bottom) Zoom-in of the Top regions outlined in black. Different protein structures are outlined in dashed colored lines. The parallel lines of density (red dashed line) show the chemoreceptor array containing McpA, the ribosome excluded regions (green dashed line) at the poles of the bacterium are the inferred position of PopZ, and the position of SpmX is undetermined in CET alone. (B) Single-color room temperature SMACM histogram reconstructions of the three proteins each separately fused to PAmKate. Cell outline manually added from bright-field images of the same region.