Fig. 5.

Induction of checkpoint molecules in response to T cell proliferation and IFN-γ production. (A) PD-L1 expression on myeloid cells (CD33⁺) from the bone marrow of patients with AML. (B) Representative PD-1 ligand staining of a proliferator sample after 5 d of culture with either mIgG + mIgG (Left) or anti-CD3 + mIgG (Right). (C and D) Box-and-whisker plots denoting medians and interquartile ranges of (C) frequency of PD-1 ligand expressing cells and (D) IFN-γ from the culture supernatants of proliferator samples, with or without TCR stimulation. P values for C and D are from two-tailed paired t test.