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. 2020 Jun 8;117(25):14395–14404. doi: 10.1073/pnas.1918596117

Fig. 4.

Fig. 4.

Enhanced RNA stability and transcriptional activation of TRIM25 mediated by RIG-I contribute to the up-regulation of TRIM25. (A) Enhancement of stability of TRIM25 mRNA in NB4 cells with ATRA treatment. (B) RIG-I enhanced TRIM25 mRNA stability in U937-RIG-I cells with RIG-I induction upon doxycycline depletion. (C) The RNA-binding domains of RIG-I stabilized TRIM25 mRNA in HEK 293T cells. (D) Schematic diagram of TRIM25 promoter and DNA fragments near the transcription initiation site of the TRIM25 gene. (E) The Luciferase assay of the transcriptional activity of the sequence containing ISRE in the first intron of TRIM25 upon treatment with ATRA in NB4 cells and RIG-I induction in U937-RIG-I cells, respectively. (F) Activation of the ISRE in the first intron of TRIM25 by the CARD domain of RIG-I in HEK 293T cells. (G) Up-regulation of STAT1 mediated by the CARD domain of RIG-I, which was capable of binding to the ISRE in the first intron of TRIM25.