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. 2020 Jun 8;117(25):14395–14404. doi: 10.1073/pnas.1918596117

Fig. 6.

Fig. 6.

The effect of ISGylation on myeloid differentiation and maturation. (A) Knockdown of TRIM25 or ISG15 in NB4 cells inhibited the ATRA-induced up-regulation of ISGylation. Immunoblotting was used to detect ISGylation expression in NB4 cells with TRIM25 or ISG15 knockdown after 3 d of treatment with 1 µM ATRA. (B) TRIM25 or ISG15 knockdown in U937-RIG-I cells inhibited ISGylation. Immunoblotting was used to detect ISGylation expression in U937-RIG-I cells with TRIM25 or ISG15 knockdown. (C) Knockdown of TRIM25 or ISG15 in NB4 cells inhibited granulocytic differentiation stained with Wright-Giemsa. (D) TRIM25 or ISG15 knockdown in U937-RIG-I cells inhibited cell differentiation as revealed by morphological examination with Wright-Giemsa staining. (E) Flow cytometric analysis of phagocytosis in NB4 cells with TRIM25 or ISG15 knockdown. NC: control group without ATRA treatment. The experimental groups were treated with 1 µM ATRA for 3 d. *P < 0.05; ***P < 0.001.