Figure 2.
KZ-02 Exhibits Higher Activity Than AZD6244 in Inhibiting CRC Growth
(A and B) Examination of activity of KZ-02 versus AZD6244 in CRC cell growth inhibition. Colo205 (A) or HT29 (B) cells were treated with increasing concentrations of KZ-02 or AZD6244 and cell proliferation was determined at 24, 48, and 72 h by using the MTT method. Error bars are based on the standard deviations of triplicate samples.
(C) IC50 values for KZ-02 and AZD6244 calculated from data shown in (A) and (B), and the ratio of their IC50s was also calculated.
(D) Examination of antitumor effects of KZ-02 versus AZD6244 in xenograft models. BALB/c mice were subcutaneously injected with 1×107 Colo205 cells. Mice were then randomly assigned to three groups (n = 8), vehicle, KZ-02, and AZD6244, which were orally administered once daily for 20 days. Tumor size and body weight were measured every 3 days from day 23 to day 41.
(E) Body weight variations of mice used in (D).
Data are represented as mean ± SD. Comparisons of two groups are performed with Student's t test. ∗p < 0.05, ∗∗∗∗p < 0.0001.