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. 2020 Jun 10;23(7):101254. doi: 10.1016/j.isci.2020.101254

Figure 3.

Figure 3

KZ-02 Additionally Targets Pim-1

(A) Kinase selectivity screening identified Pim-1 as an additional target for KZ-02 with IC50 being 1.34 μmol/L.

(B) Examination of KZ-02 inhibition on Pim-1 activity. Colo205 and HT29 cells were treated with KZ-02 (10 nmol/L) or DMSO (control) for 2 h, followed by western blot analysis. Cdc25C is a known substrate of Pim-1.

(C) Either Pim-1 1, a Pim-1 inhibitor, or Pim-1 siRNA treatment decreased Cdc25C phosphorylation. Cells were treated with 100 nmol/L Pim-1 1 for 2 h, or 40 nmol/L Pim-1 siRNA for 48 h, and then were lysed and subjected to western blot analysis. Data are represented as mean ± SD.