Figure 3.

HFD predominately alters PUFA species of phospholipids and ether lipids, and these are variously impacted by short-term inhibition of autophagy. Mice were dieted and treated with tamoxifen for 3 weeks as indicated in the legend to Figure 1. Islets were isolated and total cellular lipids extracted and quantified by MS. Data are from five to seven independent values. Individual lipid species were tested for significant modulation by HFD (P < 0.05 unpaired t-test, corrected for multiple comparison with 0.05 false discovery rate. (A) Shown are significantly altered species categorized by (A) lipid class or (B) side-chain. (C) Cluster analysis (Pearson correlation) and heat map (Log2 and row adjusted) of significantly altered species: i) decreased by HFD partly reverted by ATG7 deletion ii) increased by HFD independently of ATG7 deletion, and iii) decreased by HFD independently of ATG7 deletion.