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. 2020 Jun 3;40:101023. doi: 10.1016/j.molmet.2020.101023

Figure 7.

Figure 7

Ether lipid accumulation in MIN6 cells is preferentially inhibited by pre-treatment with oleate compared to palmitate. MIN6 cells were cultured for 48 h with 0.4 mM oleate or palmitate complexed to 0.9% BSA. Total cellular lipids were extracted from cells and quantified by MS. Data are means ± SEM of four independent values. Shown are summed masses of total species of (A) ether lipids, or (B) canonical phospholipids, expressed relative to the corresponding BSA control. (C) Individual species of ether lipid significantly modulated by FA pre-treatment (P < 0.05 unpaired t-test, corrected for multiple comparison with 0.05 false discovery rate), are presented using cluster analysis (Pearson correlation) and heat map (Log2 and row adjusted). (D–F) Mass of major species of SFA- and MUFA-containing alkyl lipids (D), plasmalogens (E), or PUFA-containing plasmalogens (F) are shown per mg of cellular protein. Statistical analyses were by two-way ANOVA with Tukey post hoc test. #P < 0.05, ###P < 0.0005, ####P < 0.0001 versus chow control, or ∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.0005 as indicated.