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. 2020 Jun 12;23(7):101265. doi: 10.1016/j.isci.2020.101265

Figure 2.

Figure 2

Punctate ERESs Occur as Golgi-Associated and Golgi-Free ERESs

This figure shows VAEM images of the surface of cotyledon epidermal cells of transgenic plants. Scale bars, 3 μm.

(A) Representative images of MAG5-GFP (ERES marker) and mCherry-SYP32 (Golgi marker). Circles indicate beaded rings of ERESs surrounding Golgi stack (Golgi-associated ERESs), and arrowheads indicate individually distributed ERESs free from Golgi stacks (Golgi-free ERESs).

(B) Proportions of Golgi-associated ERESs and Golgi-free ERESs to total MAG5-GFP-labeled punctate ERESs. Error bars represent 95% confidence intervals. n = 1,066 MAG5-GFP-labeled punctate ERESs from five biological replicates. See also Figure S2 for image processing procedures for extraction of Golgi-free ERESs and Golgi-associated ERESs.

(C) Time series of images of MAG5-GFP (ERES marker) and mCherry-SYP32 (Golgi marker) for 4.0 s. See also Video S1 for the original real-time movie.

(D) Time series of images of mCherry-SYP32 (Golgi marker) and each of COPII markers, SEC13A-GFP (upper panels) and YFP-SEC24A (lower panels), for 4.0 s.