Figure 5.

Punctate-ERES Cycling Is Independent of a Cytoskeleton-Dependent Movement

(A) Time series of representative VAEM images of MAG5-GFP (ERES marker) and mCherry-SYP32 (Golgi marker) for 5.5 s. The cotyledon cells were mock-treated (upper panels) or treated with cytoskeleton inhibitors (10 μM latrunculin B and 50 μM oryzalin) (lower panels) for 60 min. See also Video S5 (left and right) for the original real-time movies of mock- and inhibitor-treated cells, respectively. Scale bars, 3 μm.

(B and C) Six example trajectories of fluorescence particles corresponding to each of Golgi stack, Golgi-associated ERES, and free ERES in cotyledon cells that expressed MAG5-GFP (ERES marker) and mCherry-SYP32 (Golgi marker). The cotyledon cells were mock-treated (B) or treated with the cytoskeleton inhibitors (C) for 60 min. Time-coded trajectories of the fluorescence particles were tracked from the center of each two-dimensional lattice. Three biological replicates were performed.