Figure 2.
Tau-Immunoreactive Clusters Are Reduced, but Not Eliminated, in Tau KO Mice When Detected with Pan-Reactive Secondary Antibodies
(A) Immunofluorescent confocal images of aged (23-mo) wild-type (WT) and tau knockout (TKO) mice show immunoreactivity with K9JA (red) and tau-1 (green) in the CA1 and SR of WT mice, with only tau-1IR cluster immunoreactivity in TKO mice.
(B) Representative images of full hippocampal fields in aged WT and TKO mice captured by confocal microscopy at 40x magnification.
(C) Quantification of tau-1IR cluster density showing a reduction in granules in 23-mo TKO mice when compared with age-matched WT mice.
(D) Immunoblots of tau (tau-1, K9JA) and GAPDH in 23-mo WT and TKO mouse hippocampal lysates confirm the absence of tau expression in TKO mice.
Scale bars, 50 μm (A), 250 μm (B). Statistical significance was assessed using a Student's t test from N = 4 biological replicates (∗p < 0.05). Error bars represent SEM. See also Figure S2.