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. 2020 Jun 8;117(25):14259–14269. doi: 10.1073/pnas.1913469117

Fig. 5.

Fig. 5.

O-GlcNAcylation at LATS2 Thr436 suppresses its kinase activity. (A–F) Wild-type (WT) LATS2 or LATS2 mutants were stably expressed in LATS1/2-dKO HEK293A cells. (A) Cell lysates were immunoblotted with the relevant antibodies. (B) The phosphorylation levels at YAP Ser127 were normalized to the YAP level, and the YAP expression level was normalized to the GAPDH level. (C) Phosphorylation levels at TAZ Ser89 were normalized to the TAZ level, and the TAZ expression level was normalized to the GAPDH expression level. (D) CTGF and CYR61 mRNA levels were determined by qRT-PCR. All values were normalized to the GAPDH mRNA level. (E) Lysates of the indicated Flag-LATS2 stably expressing LATS1/2-dKO HEK293A cells were immunoblotted with anti-CYR61 and anti-CTGF antibodies. CTGF and CYR61 expression levels were normalized to β-actin expression level. (F) NF2 mRNA level was determined by qRT-PCR. The NF2 mRNA level was normalized to GAPDH mRNA level. (B–F) Data are presented as mean ± SEM from at least three independent experiments. Statistical significance was determined by one-way ANOVA (*P < 0.05, **P < 0.01, ***P < 0.005).