Table 3.

Design of Experiments for the optimized chromatographic parameters.

Std	Run	Factor1	Factor2	Factor3	Response1	Response2	Response3
		Flow rate	Column Temperature	Buffer pH	Resolution btw DSA & CTZ	Resolution btw CTZ & HCTZ	Resolution btw TMS C & Chloro analog
14	5	0.8	50	3.5	2.5	2.8	2.9
13	6	0.8	50	3.5	2.5	2.8	2.9
10	9	0.8	30	3.5	2.8	3.1	2.9
6	12	0.8	50	2.5	2.4	2.9	2.9
5	14	0.8	50	2.5	2.4	2.9	2.9
2	16	0.8	30	2.5	2.8	3.3	3.1
9	17	0.8	30	3.5	2.9	3.2	3.1
1	18	0.8	30	2.5	2.8	3.1	2.8
17	3	1	40	3	2.6	2.9	2.9
18	4	1	40	3	2.6	2.9	2.8
15	1	1.2	50	3.5	2.3	2.6	2.9
3	2	1.2	30	2.5	2.4	2.8	2.8
8	7	1.2	50	2.5	2.3	2.5	2.9
11	8	1.2	30	3.5	2.4	2.8	2.9
4	10	1.2	30	2.5	2.5	2.9	2.7
7	11	1.2	50	2.5	2.4	2.6	2.8
16	13	1.2	50	3.5	2.4	2.6	2.8
12	15	1.2	30	3.5	2.5	2.9	2.9