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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Anesthesiology. 2020 Jun;132(6):1540–1553. doi: 10.1097/ALN.0000000000003241

Figure 3. Transcriptional increases of macrophage markers in DRGs of paclitaxel-treated mice after local sympathectomy.

Figure 3.

(A) Representative images and quantification of ionized calcium binding adaptor molecule 1 (IBA1) protein levels in DRG tissues of male mice with sham- and local microsympathectomy (mSYMPX) at day 7 after paclitaxel (n=6 male mice/group, t-test, p = 0.107 compared to sham). (B) The same tissues and conditions were also probed for transcriptional changes and similarly to the protein levels, the IBA1 mRNA was unchanged (n=4 male mice/group, t-test, p = 0.141 compared to sham). (C) In contrast, the macrophage markers cluster of differentiation 68 (CD68), EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1), integrin alpha X (ITGAM) and C-X3-C motif chemokine receptor 1 (CX3CR1), as well as the monocyte chemotactic markers chemokine (C-C motif) ligand 2 (CCR2) and C-C chemokine receptor type 2 (CCL2) were significantly increased (n=4 male mice/group, t-test, *p<0.05, ***p < 0.001 compared to sham).