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. 2020 Jun 7;23(6):101248. doi: 10.1016/j.isci.2020.101248

Figure 3.

Figure 3

BATTLE-2.1: Triple Splitting Allocation of Transgenes Using the Battle of the Recombinases

(A) In BATTLE-2.1 technology, Cre, FLPO, and Dre are expressed under the control of CamKIIa promoter. Cre excises FLPO and Dre transgenes, whereas FLPO excises Cre and Dre transgenes. Dre excises FLPO and Cre transgenes. Using AAVs, Cre induces mCherry expression and FLPO induces YFP expression. In addition, Dre induces mTFP expression.

(B) A representative maximum intensity projection image of hippocampus infected with the BATTLE-2.1 system when the ratio of Dre:Cre:FLPO was 1:1:1. Scale bar, 100 um.

(C) Magnified maximum intensity projection image (6 μm thickness) of the boxed area in (B). Red color shows mCherry expression.

(D) Magnified maximum intensity projection image of the boxed area in (B). Yellow color shows YFP expression.

(E) Magnified maximum intensity projection image of the boxed area in (B). Blue color shows mTFP expression.

(F) Superposed maximum intensity projection image of the boxed area in (B). Red, yellow, and blue show mCherry, YFP and mTFP, respectively. Scale bar, 10 μm.