Skip to main content
. 2020 Jun 7;23(6):101248. doi: 10.1016/j.isci.2020.101248

Figure 4.

Figure 4

BATTLE-1EX: Three-Dimensional (3D) High-Resolution Imaging of Whole Synaptic Structures

(A) (Top) Illustration of DG-CA3 synapse visualized using AAV-CAG-GFP. (Left) Representative maximum intensity projection image in the CA3 region infected with conventional virus AAV-CAG-GFP. (Right) Magnified confocal image of boxed area to the left. Note unclear morphologies of whole synaptic structures of DG-CA3 synapses.

(B) (Top) Illustration showing BATTLE-1EX. BATTLE-1-infected slices are expanded using 4.1× expansion microscopy (ExM). (Bottom) Representative maximum intensity projection image of CA3 stratum lucidum of BATTLE-1EX slices.

(C) (Top left) Magnified maximum intensity projection image of boxed area in (B). (Top, right) Magnified confocal images of boxed area in (C) top left.

(D) Cross-sectional line profiles of fluorescent intensities in boxed region in (C) right.

(E) Representative volume-rendering 3D image of the whole synaptic structure of DG-CA3 synapses. Scale bar, 732 nm.

(F) Same volume-rendering 3D image in (D) without YFP.

(G) (Left) Magnified images of (E). (Right) the sequential z stack images of the boxed area in (left). Scale bar, 500 nm.