Figure S4.
Trypsin protection assay shows that PyoG1-485 AF488 gets translocated across the outer membrane. (A) Representative micrographs of cells treated with trypsin after labeling with fluorescent PyoG. Cells remain fluorescent after trypsin treatment, meaning that PyoG is not available for degradation on the cell surface, but protected from trypsin due to import. (B) Average fluorescence intensities of 100 cells in the presence and absence of trypsin and fluorescent PyoG. Mean of three biological replicates with standard deviations is shown.