Figure 3.

Tidal activity rhythm in E. pulchra generates antisense tidal cycles of metabolism but is independent from classical clock genes oscillation. (A) Swimming activity pattern of Eurydice specimens shows a period of 12.5 h in free-running conditions, with maxima during predicted high water (HW). (B) Representative western blot of overoxidized peroxiredoxin. (C) Relative intensity of overoxidized head peroxiredoxin. (D and E) Relative expression of mitochondrially-encoded genes (ND = NADH dehydrogenase subunits, COX = cytochrome oxidase subunits; CYTB = cytochrome B) under free-running laboratory conditions. (F) None of Eurydice circadian clock genes exhibit a tidal rhythm at the transcript level, and curiously, only tim shows a clear circadian profile (red arrows show the time of expected high water). For references, see [10,54]. Orange circles: artificial (lab) conditions.