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. 2020 Apr 7;29(10):1635–1647. doi: 10.1093/hmg/ddaa059

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Glial activation in thalamic VPL/VPM, VM and VL regions of Ngly1^−/− rats. Immunohistochemistry for GFAP in thalamus of WT and Ngly1^−/− rats at 5 and 29 weeks of age, showing an increase in area of reactive astrocytes. (A, B) Immunohistochemistry for GFAP (A) and IbaI (B) in the thalamus of WT and Ngly1^−/− rats at 5 weeks of age. Scale bar 2.5 mm. Arrows indicate the regions with gliosis in Ngly1^−/− rats. Nuclei were stained with DAPI. (C, D) Quantitative analyses show the area occupied by the GFAP (C) or IbaI (D) positive areas in thalamus of WT and Ngly1^−/− rats. Values represent mean ± SEM (n = 6). Asterisks indicate ^*^*P < 0.01.