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Changes of AMPK, mTOR, and ULK1 expression during late exercise preconditioning. (a–e) Images and quantitative analysis of AMPK, AMPKαThr172, mTOR, ULK1, and ULK1Ser757 in rat myocardium by western blot assay. (f) Quantitative analysis of ULK1Ser757/ULK1. ∗P < 0.05 vs. C. (g) Autophagosomes and lysosomes were observed from these groups by TEM. (×5,000), bar = 1.0 μm.
