Table 1.
Effects of mesenchymal stromal cells from adipose tissue (AD-MSCs) on the mitochondrial function of permeabilized cardiac fibers (BZ) from rats after acute myocardial infarction (AMI). Rats were treated with AD-MSCs or PBS after AMI, and different parameters of the mitochondrial function of permeabilized cardiac fibers were analyzed by high-resolution respirometry, according to the multiple substrate-uncoupler-inhibitor titration protocol.
| Mitochondrial function parameter (in pmol O2 s−1 mg−1) | Necrotic phase | Fibrotic phase | ||
|---|---|---|---|---|
| PBS (n = 10) |
AD-MSC (n = 7) |
PBS (n = 7) |
AD-MSC (n = 8) |
|
| CI | 19.12 ± 1.95 | 19.14 ± 4.93 | 16.97 ± 3, 42 | 13.33 ± 2.16 |
| CIp | 120.98 ± 11.40 | 113.0 ± 17.90 | 77.65 ± 12.86 | 97.79 ± 18.31 |
| CI + CIIp | 238.72 ± 30.61 | 249.02 ± 31.95 | 185.57 ± 37.49 | 229.50 ± 42.83 |
| Leak state (L) | 102.5 ± 13.61 | 119.9 ± 12.61 | 110.86 ± 22.99 | 120.18 ± 20.10 |
| (CI + CIIp − L) | 136.19 ± 19.59 | 129.11 ± 20.1 | 74.71 ± 16.19 | 109.32 ± 25.21 |
| E | 270.24 ± 25.41 | 299.31 ± 22.06 | 234.79 ± 52.24 | 238.99 ± 40.95 |
| CImax | 120.52 ± 17.17 | 117.38 ± 8.89 | 91.15 ± 20.35 | 98.10 ± 20.85 |
| CIImax | 139.98 ± 17.82 | 168.23 ± 19.91 | 137.13 ± 33.29 | 132.28 ± 27.80 |
| ROX | 10.93 ± 1.48 | 12.18 ± 1.87 | 11.06 ± 1.10 | 11.28 ± 0.94 |
| Cyto C (%) | 15.30 ± 2.77 | 14.50 ± 1.8 | 15.90 ± 3.22 | 13.0 ± 2.76 |
| L/E | 0.37 ± 0.003 | 0.39 ± 0.01 | 0.509 ± 0.039 | 0.512 ± 0.04 |
| (CI + CIIp–L)/E | 0.50 ± 0.05 | 0.42 ± 0.05 | 0.37 ± 0.05 | 0.42 ± 0.05 |
| RCR | 2.39 ± 0.16 | 2.04 ± 0.11 | 1.72 ± 0.06 | 1.85 ± 0.14 |
| OXPHOS coupling efficiency | 0.53 ± 0.03 | 0.47 ± 0.04 | 0.37 ± 0.02 | 0.41 ± 0.05 |
CI: nonphosphorylative state; CIp: phosphorylative state associated with complex I; CI + CIIp: maximal phosphorylative state; (CI + CIIp − L): flux coupled with ATP synthesis; E: maximal respiratory capacity; CImax: complex I contribution to the maximal ETS capacity; CIImax: complex II contribution to the maximal ETS capacity; ROX: residual oxygen consumption; Cyto C: an index of mitochondrial outer membrane integrity; L/E: leak control ratio; (CI + CIIp − L)/E: expressing how much from ETS capacity is used to produce ATP; RCR: respiratory control ratio. Values are expressed as the mean ± SEM. Any difference was visible between the vehicle-treated (PBS) group and the AD-MSC-treated group (by unpaired t-test).