Figure 5.

Western blot analysis of expression levels of the NF-κB signaling pathway in Hep3B and HepG2 liver cells. The protein expressions of p65, p-p65, IκBα, and p-IκBα in Hep3B and HepG2 liver cells measured by Western blotting. Results revealed that the expression levels of p-IκBα and p-p65 were decreased in HepG2 and Hep3B liver cancer cells treated with apatinib when compared with the control group in a dose-dependent manner. And the ratio of p-IκBα/IκBα and p-p65/p65 in cells was significantly lower compared with that of the control group. ImageJ software was used to analyze the gray values. ∗ indicates p < 0.05, ^∗∗p < 0.01.