Figure 1.

Characterization of acidic compartments in muscle cells. Chick myogenic cells were labeled with acridine orange, analyzed under a fluorescent microscope (a–d, g–l). Control (untreated) cells were grown for 24, 48, 72, or 96 hours (a–d). Other cells were grown for 24 hours, treated with DMSO, or Lys05-treated for the next 24 hours (g–l). Acidic compartments (AC) were found specially concentrated in the perinuclear area of mononucleated cells (arrow in (a)). White outline shows how the cell surface was measured (a). The area and diameter of acidic compartments were quantified (e, f, m, and n). Scale bar = 50 μm.