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Knockdown of LAMP-2 protein inhibits chick myogenesis. Chick myogenic cells were transfected with siRNA against chick LAMP-2 or eGFP and double-labeled for desmin and DAPI (a–d). Scale bar in (d) = 50 μm. Transfected cells were also submitted to Western blotting for LAMP-2 (f). Note that LAMP-2 knockdown induces a significant decrease in acidic compartment (AC) size, muscle proliferation, and differentiation (e, g).
