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Activation of the Wnt/beta-catenin pathway rescues the effects of acidic compartment inhibition. Chick myogenic cells were treated with either DMSO, Lys05, BIO, or Wnt3a and labeled with acridine orange (a–g). Scale bar in (a) = 10 μm. The area and diameter of acidic compartments (AC) were quantified (h). Cells were also labeled for desmin and DAPI, and several parameters of muscle proliferation and differentiation were quantified (i).
