Fig. 2.

Spectra of myoglobin during in vitro digestion. (A) 279 nm bands of UV–Vis spectra; (B) 409 nm bands of UV–Vis spectra; (C) Intrinsic fluorescence spectra of Mb; (D) Synchronous fluorescence spectra for Tyr residues (Δλ = 20 nm); (E) Synchronous fluorescence spectra for Trp residues (Δλ = 60 nm); (F) CD spectra of myoglobin. G0, G3, G60 and G120 refer to pepsin-treated samples for 0, 3, 60 and 120 min, respectively; I1, I3, I15, I60 and I120 refer to pancreatin-treated samples for 1, 3, 15, 60 and 120 min after treatment by pepsin, respectively.