Figure 5.

bFGF promoted SHEDs proliferation. Colony formation was stained with Coomassie blue at day 14 (A and B). In some conditions, cells were pretreated with an FGFR or PI3K inhibitor for 30 min prior to bFGF exposure. Further, the cells were maintained with or without the inhibitor and bFGF for 14 days. Black bars in (B) indicate 200 μm. Cells were treated with bFGF for 72 h and subsequently stained with PI/RNase. Cell cycle analysis was performed using flow cytometry. DNA histograms (C). The percentage of cell cycle subpopulations (D). Asterisks indicate a significant difference compared with the same subpopulation group in the control.