Fig. 2.

GTS-21 inhibits hyperoxia-induced translocation and accumulation of HMGB1 in the mouse airways and circulation. C57BL/6 mice were exposed to either 21% O₂ (room air) or to ≥99% O₂ and treated with vehicle (0 mg/kg) or GTS-21 (4 mg/kg), as described in Fig. 1. Bronchoalveolar lavage, whole blood, and lungs were harvested at the end of hyperoxia exposure. a A representative image of Western blots showing immunoreactive bands of HMGB1 in the mouse BAL samples. The bar graph shows the integrated density value of the HMGB1 bands of the BAL of hyperoxic mice, administered with normal saline or GTS-21 (n = 10 mice/group). b A representative Western blot for HMGB1 in mouse serum and the integrated density values for each group (N.D., not detected) (n = 3 mice/group). c Representative immunohistochemical staining for HMGB1 in mouse lungs (arrows indicate HMGB1 in nucleus, asterisks represent HMGB1 present in cytoplasm) (n = 3 mice/group). *p < 0.05, compared with mice receiving normal saline