Fig. 2.

Examples for non-immunoaffinity methods to improving sensitivity for LC-MS-based quantification of antibodies from plasma or tissue homogenates. The detailed procedures can be found in the corresponding publications. (A) General workflow of a selective antibody free, peptide-level CX-RP enrichment, to improve sensitivity for LC-MS quantification. Adapted with permission from Ref. [107]. Copyright (2020) American Chemical Society. (B) A trapping-micro-LC-MS workflow for quantitative analysis of mAb with high sensitivity, exceptional robustness and high throughput. Reprinted with permission from Ref. [108]. Copyright (2020) American Chemical Society.