Fig. 2.

Ginsenoside Mc1 exhibited a protective effect after the treatment of H9c2 cells with hydrogen peroxide (H₂O₂). (A and B) Cells were preincubated with ginsenoside Mc1 (25 or 50 μg/mL) or ginsenoside Mc1 plus compound C (2 μM) for 24 h and then stimulated with H₂O₂ (600 μM) for 2 h. Bax and Bcl2 levels were determined by Western blotting. (C and D) H9c2 cells were pretreated with ginsenoside Mc1 or ginsenoside Mc1 plus compound C for 24 h and then stimulated with H₂O₂ for 4 h. Cell viability was measured using an EZ-CYTOX kit. The mean ± standard deviation was obtained from 3 separate experiments [*, p < 0.05; **, p < 0.005; ***, p < 0.0005; analysis of variance (ANOVA)]. Bax, Bcl2-associated X protein.