Figure 2.
Mitochondrial function and redox cycle alteration in 2D and Agg-D hMSCs. (A): Representative images of mitochondrial morphology in 2D vs. Agg-D hMSCs, stained with Mitotracker Red, taken at ×100. Scale bar is 50 μm. (B): Circularity of mitochondria in 2D vs. Agg-D hMSCs. (C): ATP content with or without 2DG treatment in 2D vs. Agg-D hMSCs. (D): Mitochondrial membrane potential measured by flow cytometry, (E): electron transport complex I activity, (F): NADH level and (G): NADPH level in 2D vs. Agg-D hMSCs. *, p<0.05; **, p<0.01.