Fig. 3.

PRKCQ regulates chemotherapy sensitivity by modulating Bim expression. a Basal levels of Bcl2 family proteins were assessed in immortalized MCF-10A breast epithelial cells overexpressing vector control or PRKCQ (kinase-active or kinase-dead). b Levels of Bim transcript were assessed in MCF-10A breast epithelial cells overexpressing vector control or kinase-active PRKCQ by TaqMan assay. Fold change in Bim transcript levels was calculated with normalization to either of two control housekeeping transcripts (Beta-2 microglobulin or GAPDH). c–e Bim protein expression in MDA-MB 231-luc and HCC1806 cells was assessed 48 h, 72 h, and 96 h post-infection with PRKCQ shRNA-expressing lentivirus. f Levels of Bim EL transcript were determined in MDA-231Luc cells expressing control or PRKCQ shRNA 54 using Bim EL-specific primers, as indicated in the “Methods” section