Fig. 4.

Bim levels determine chemotherapy sensitivity. Levels of apoptosis were assessed following 24 h paclitaxel treatment of MCF-10A cells expressing control or two different Bim shRNA vectors by Western analysis for cleaved PARP (a) or Caspase Glo® 3/7 Assay (b). c Levels of cleaved PARP were assessed in MDA-MB231 Luc cells co-expressing PRKCQ and Bim shRNA following 24 h treatment with vehicle control or paclitaxel. Western blots are representative of at least 3 independent experiments. d Apoptosis in MDA-MD231 Luc cells expressing PRKCQ shRNA +/-Bim siRNA and treated with DMSO or paclitaxel was assessed using Caspase Glo® 3/7 Assay. Fold change in apoptosis relative to vector/siRNA control-expressing cells treated with DMSO is shown. Results are averages of at least 3 independent experiments. Significance was determined using Student’s t test. NS, not significant. *p < 0.05; **p < 0.01; ***p < 0.001. LE, light exposure; DE, dark exposure