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. 2020 Jun 29;22:72. doi: 10.1186/s13058-020-01302-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — A novel PRKCQ small-molecule kinase inhibitor (17k) suppresses growth and enhances apoptosis of chemotherapy-treated TNBC cells. a Treatment of MDA-MB 231-luc or HCC1806 cells with 17k suppresses autophosphorylation of PRKCQ at Threonine 538. Cells were treated at the indicated concentrations for 4 h. 17k treatment suppresses growth in 3D Matrigel culture (b), suppresses invasion in Transwell Matrigel assays (c), increases anoikis (24 h in suspension cultures) (d), and induces Bim expression in suspension cultures (e). 17k pre-treatment (48 h) enhances paclitaxel-induced death as measured by the CellTiter-Glo® 3D Luminescence Cell Viability Assay in MDA-MB 231-luc cells (f) and by Annexin V+ staining in HCC1806 cells (g). For CellTiter-Glo assays, percent viability is plotted relative to DMSO control (set at 100%). All experiments were repeated a minimum of 3 times each. Significance was determined using Student’s t test