Fig. 2.

NET formation is largely dependent on platelets in sepsis. WT mice were treated with busulfan (25 mg/kg, i.p.) or anti-platelet Ab (50 μL/mouse, i.v.) prior to CLP. a, b Quantification of dsDNA and circulating NET structures in the plasma of mice using PicoGreen fluorescent dye and MPO-DNA-ELISA, respectively. c Quantification of dsDNA in BALF using PicoGreen fluorescent dye. d Exosomes were isolated from equal plasma volumes, and the protein concentration was determined using a BCA protein assay kit. e Plasma NETs and exosomal protein concentration are positively correlated (r = Pearson’s correlation coefficient). f At 24 h after sham or CLP, the wet/dry ratio of lung tissue was measured. g The total protein concentration in BALF was quantified using BCA. h Lung tissues were harvested at 24 h after CLP. Supernatant was made after homogenation and centrifugation. Equal amount of supernatant was spread on agar plates for colony formation. The number of bacterial colonies was assessed. Graphs represent means ± SEM, n ≥ 3; **P < 0.01 compared within two groups