Table 3.
Free AA concentrations in refed dam mammary tissue on LD131,2
| Amino acid | AP | PR | AP-R | |||
|---|---|---|---|---|---|---|
| μmol/g protein | μmol/g protein | P-value3 | μmol/g protein | P-value3 | SEM | |
| EAA | ||||||
| Arginine | 1.98 | 3.57 | 0.61 | 1.88 | 0.98 | 1.91 |
| Histidine | 7.13 | 5.18 | 0.83 | 2.12 | 0.73 | 4.04 |
| Isoleucine | 4.31 | 4.84 | 0.82 | 4.50 | 0.97 | 1.05 |
| Leucine | 3.87 | 22.0 | 0.73 | 20.1 | 0.77 | 28.1 |
| Lysine | 14.7 | 16.8 | 0.88 | 17.8 | 0.83 | 5.40 |
| Phenylalanine | 2.09 | 2.88 | 0.69 | 1.58 | 0.84 | 1.10 |
| Threonine | 6.48 | 7.13 | 0.91 | 9.04 | 0.36 | 1.95 |
| Valine | 2.71 | 3.20 | 0.91 | 3.26 | 0.89 | 1.42 |
| NEAA | ||||||
| Alanine | 23.5 | 43.7 | 0.28 | 58.2 | 0.05 | 14.1 |
| Aspartate | 1.98 | 2.74 | 0.72 | 3.17 | 0.48 | 1.16 |
| Glutamate | 20.2 | 23.3 | 0.84 | 23.2 | 0.85 | 6.62 |
| Glutamine | 7.06 | 14.7 | 0.08 | 15.5 | 0.05 | 3.53 |
| Proline | 3.19 | 5.33 | 0.38 | 5.50 | 0.33 | 1.75 |
| Serine | 8.42 | 12.8 | 0.37 | 7.42 | 0.93 | 3.52 |
| Tyrosine | 5.39 | 5.67 | 0.97 | 6.25 | 0.81 | 1.64 |
1AP = 18% crude protein diet, PR = 9% crude protein diet, AP-R = 18% crude protein diet coupled with rapamycin injections
2Data are means and pooled SEM, n = 6/treatment
3P-values correspond to difference between respective treatment and AP by ANOVA with Dunnett post hoc