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. 2020 Jun 26;48(6):0300060520931242. doi: 10.1177/0300060520931242

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© The Author(s) 2020

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — Morphological analysis (first line, ×100) and chemical staining (second line, ×400) of NFs and co-culture with NFs from colon cancer (a). NFs showed fusiform or star-shaped morphology of the same size, while after co-culture, the morphology of the NFs was fusiform or a polygon with different sizes. This could be observed in the binuclear and polynuclear cells, while α-SMA expression was negative in NFs and positive in co-culture NFs. α-SMA protein expression in NFs and co-cultured NFs from colon cancer was determined using SDS-PAGE and an ODYSSEY fluorescence imaging system (b); calculations were performed using ImageJ image analysis software (c); and the cellular immunofluorescence results are presented (d).

NF, normal fibroblasts; α-SMA, α-smooth muscle actin; SDS-PAGE, sodium dodecyl (lauryl) sulfate-polyacrylamide gel electrophoresis.