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. Author manuscript; available in PMC: 2021 May 13.
Published in final edited form as: J Am Chem Soc. 2020 May 4;142(19):8848–8861. doi: 10.1021/jacs.0c01881

Figure 3. Precipitates formed by an S:H:L ternary mixture.

Figure 3.

(a) Brightfield image of the precipitates at 25 minutes after mixing S, H, and L at 10, 40, and 150 μM, respectively. (b) Enlarged view of a region inside the precipitates, visualized by fluorescence of Alexa 594-SH35 (red), FITC-heparin (green), and Cy5-lysozyme (magenta). The last panel shows the merge of the green and magenta channels. (c) ThT binding (yellow) to S:H:L precipitates formed from S:H:L = 10:40:150 μM and labeled with FITC-heparin and Cy5-lysozyme, imaged at 5 min after mixing. The last panel shows the merge of the green and magenta channels.