Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 10;11:154. doi: 10.1186/s13287-020-01665-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Direct reprogramming of MSCs to d-iHEPs using dox-inducible Foxa2. Schematic diagram depicting the procedure and time-course for the direct conversion of MSCs into d-iHEPs (a). Colonies of epithelial-like d-iHEPs (red dashed circles) with GFP expression are observed after 15 days of iHEP medium exposure (b). Purified d-iHEPs at passage 6 expressing the hepatic markers albumin (ALB) and CK18 (green) by immunofluorescence. Nuclei are visualized in blue by DAPI staining (c). The increased presence of fibroblast-like cells after continuous passaging in the absence of doxycycline (d). RT-qPCR showing decreased levels of Foxa2 mRNA in d-iHEPs when compared to iHEPs (e). Validation of Tet-on Foxa2 system by analysis of Foxa2 expression in iHEPs and d-iHEPs in the presence or absence of doxycycline, by RT-PCR (f). ***p < 0.001. Scale bars 50 μm