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. 2020 Apr 10;11:154. doi: 10.1186/s13287-020-01665-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Mitochondrial content reflects different subpopulations in late iHEP cultures. Phase-contrast images obtained from iHEP cultures after mitochondrial content-based cell sorting for isolation of iHEP MT^High and iHEP MT^Low supopulations. Bars 100 μm (a). RT-qPCR analysis of albumin, AFP, HNF4α, Epcam, KRT19, and Sca-1 mRNA levels, comparing the two populations (b). Relation between intensity of GFP fluorescence (Hnf4α-reporter) and MitoTracker staining. MT^High and MT^Low iHEPs were labeled with MitoTracker Red FM and analyzed by flow cytometry (c). Morphological alterations observed by phase-contrast microscopy in iHEP MT High subpopulation after being exposed to maturation media for 7 days. Bar 100 μm (d). RT-qPCR analysis for quantification of aquaporin-1, Afp, and albumin mRNAs after maturation protocols. *p < 0.05; **p < 0.01; ***p < 0.001 (e)