Figure 4.

LRP6 and GSK‐3β protein expression in callus tissue with or without intermittent parathyroid hormone treatment. Western blot of (A) low‐density lipoprotein receptor‐related protein 6 (LRP6); (B) total Glycogen synthase kinase 3 beta (GSK‐3β); (C) inactive GSK‐3β (phospho‐S9); and (D) active GSK‐3β (phospho‐Y216). Corresponding quantification is graphed underneath each plot. Data are mean ± SEM are represented as the densitometric ratio of each experimental group relative to the saline‐treated animals. n = 5‐6, P < .05, Kruskal‐Wallis test. Groups not sharing a letter (eg a, b) are significant. Groups sharing a letter (eg a, a) are not significantly different