Figure 3.

Effects of evodiamine and its derivatives on H₂O₂‐induced cytotoxicity in SH‐SY5Y cells determined by CCK‐8 assay. The SH‐SY5Y cells were seeded in 96‐well plates (10 000 cells/well) and cultured for 24 h. Evodiamine and its derivatives (41, 47, 49, 51, 54, 58, 59, 60 and 61) were then added to the wells at doses of 0.5 µg/mL, 0.05 µg/mL and 0.005 µg/mL and the cells were cultured for 3 h. H₂O₂ was then added to the wells at a final concentration of 150 μmol/L and the cells were cultured for 24 h. Cell viability was then evaluated by CCK8 assay. The values shown are means ± SE (n = 3)