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Role of betaine in inhibition of MCF-7 cell growth. 3000 MCF-7 cells were cultured with 3 ml medium per well in 6-well plates. Dose curve: ethanol (50 mM) and different amounts of betaine were added into the medium at the 0 hour, the 48th hour and the 96th hour, respectively. The pictures were taken under a microscope at the 6th day. Original magnification X 40.
