Fig 3.
The role of betaine in MCF-7 cell growth by MTT assay. Approximately 300 MCF-7 cells were cultured with 300 μl medium per well in 96-well plates. (A) Dose curve. The ethanol (50 mM) and different concentrations of betaine were added into the medium at the 0 hour, the 48th hour and the 96th hour, respectively. OD value was detected after treated the cells for 6 days. (B) Time curve. The concentration of ethanol and betaine were 50 mM and 40 mM, respectively. OD value was detected every day after plating.