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Colony formation assay. 1000 MCF-7 cells were planted in the upper agar in 6-well plates. Ethanol (50 mM) and betaine (40 mM) were added at the 1st and 4th, respectively. The result indicates that betaine is able to reduce the rate of MCF-7 cell colony formation caused by ethanol. *P < 0.01; **P < 0.01.
