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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Chem Biol Interact. 2020 May 11;325:109129. doi: 10.1016/j.cbi.2020.109129

Fig 5.

Fig 5.

Betaine represses Brf1 expression. MCF-7 cells were pre-treated with betaine for 2 hours and then treated with ethanol for another 1 hour. (A) Immunoblot analysis was performed using protein lysates derived from these cells to detect the Brf1 protein level. Actin was used as a loading control. A represent immunoblot (A, Left panel). A densitometric analysis are revealed and the fold changes in Brf1 level were normalized by actin (A, Right panel). (B) Brf1 mRNA level. MCF-7 cells were treated with alcohol (50mM) and betaine as indicated above, to extract total RNA. mRNA levels of Brf1 were measured by RT-PCR. The fold changes are calculated by normalizing to the amount of GAPDH mRNA. The bars represent Mean ± SE of at least three independent determinations. These results indicate that betaine reduces the induction of Brf1 expression caused by alcohol.