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. Author manuscript; available in PMC: 2021 Jul 27.
Published in final edited form as: Neurosci Lett. 2020 May 1;732:135017. doi: 10.1016/j.neulet.2020.135017

Figure 2. Time to motor impairments and paralysis in the M83+/− IM seeding model is significantly delayed in the context of hPFFs comprised of C-terminally truncated or Ser129 phosphomimetic αsyn indicative of strain-like differences.

Figure 2.

(A-F) Cohorts of M83+/− αsyn transgenic mice (n=9-12 per hPFF type; also see Table 1) were unilaterally injected with 5 μg of hPFFs (or molar equivalent for truncated forms) into the gastrocnemius at 2 months old and time to disease onset was monitored; survival curves for all hPFFs were compared with FL αsyn hPFFs (statistical details see Table 2). Mice were injected with hPFFs comprised of (A) 1-115 αsyn or 1-115 and FL αsyn in a 1:1 ratio, (B) 1-119 αsyn or 1-119 and FL αsyn in a 1:1 ratio, (C) 1-122 αsyn or 1-122 and FL αsyn in a 1:1 ratio, (D) 1-125 αsyn or 1-125 and FL αsyn in a 1:1 ratio, (E) 1-129 αsyn or 1-129 and FL αsyn in a 1:1 ratio, or (F) S129E αsyn. Details of injections and statistical summary of presented data see Tables 1 and 2.