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. Author manuscript; available in PMC: 2021 Jul 27.
Published in final edited form as: Neurosci Lett. 2020 May 1;732:135017. doi: 10.1016/j.neulet.2020.135017

Figure 6. Phosphomimetic S129E αsyn alters both heterotypic and homotypic seeding and aggregation in cultured HEK293T cells.

Figure 6.

(A) Western blots displaying Triton X-100 soluble and insoluble fractions for HEK293T cells transfected to express either WT αsyn or S129E αsyn (n=6) followed by treatment with WT 21-140 αsyn or S129E 21-140 αsyn hPFFs as indicated; control transfections with no added hPFFs are also displayed. Antibody SNL4 against residues 2-12 was utilized for detection of transfected αsyn. The presence of S129E in the PFFs or cellularly expressed human αsyn decreases prion-like seeding and aggregation propensity; S129E homotypic seeding and aggregation is further attenuated. The mobility of molecular mass markers (kDa) are displayed on the left. (B) Densitometric analysis of the blots in A (n=6, error bars = s.d.); one-way ANOVA with Dunnet’s test determined the seeding and aggregation efficiency for each PFF and transfection combination relative to FL αsyn treated with 21-140 αsyn PFFs. *** = p ≤ 0.001, **** = p ≤ 0.0001.